야생조류 매개 혈액 병원체용 고속 플랫폼(OpenArray) qPCR 검증

Wild birds migrating between countries can carry ticks or tick-borne Revised January 3, 2026 Accepted January 5, 2026 pathogens, making them potential vectors for disease spread. This study evaluated and compared the performance of high-throughput platform (HTP)-based *Correspondence: Jae-Ik Han diagnostic techniques for four bloodborne pathogens potentially transmitted by jihan@jbnu.ac.kr wild birds. After evaluating the performance (limit of detection [LOD], coefficient Funding None. of determination [R2], PCR efficiency) of the selected singleplex qPCR assay for each pathogen, each assay that met the criteria was selected and examined to verify its performance on the HTP-based chip. The test results indicated that the singleplex qPCR assay had an LOD of 1 × 101-1 × 102 molecules/µL, R2 greater than 0.98, and acceptable PCR efficiency, comparable to the performance standards for conventional multiplex qPCR assay on HTP-based chips. The four qPCR assays investigated in this study are expected to be included as individual assays in HTP-based chips and will be utilized in a national-scale screening system for bloodborne infectious diseases in wild birds.